Journal: Biomedicines

Article Title: Mitochondrial Respiration of Platelets: Comparison of Isolation Methods

doi: 10.3390/biomedicines9121859

Figure Lengend Snippet: Scheme of platelets (PLT) preparation using density gradient centrifugation (DC). From the first centrifugation of whole blood with Ficoll-Paque TM to the final pellet, which was resuspended in Dulbecco’s phosphate-buffered solution (DPBS) and ethylene glycol-bis (2-aminoethyl ether)- N , N , N ′, N ′-tetra-acetic acid (EGTA) at room temperature (RT). The PLT suspension was counted (10 times diluted) and added to the O2k-chamber at a final cell concentration of 200 × 10 6 x·mL −1 . PBMC, peripheral blood mononuclear cell.

Article Snippet: Calcium-free Dulbecco’s phosphate-buffered solution (DPBS) was obtained from Lonza, Switzerland.

Techniques: Gradient Centrifugation, Centrifugation, Concentration Assay

Journal: Modern Technologies in Medicine

Article Title: The Model of an Ischemic Non-Healing Wound: Regeneration after Transplantation of a Living Skin

doi: 10.17691/stm2023.15.5.01

Figure Lengend Snippet: Problems in preclinical studies of biomedical cell products arising during modeling an ischemic non-healing wound and ways to solve them in the proposed model

Article Snippet: On days 3-5, the wounds of mice in the control, scaffold, and LSE groups were washed with a sterile 0.1% solution of gentamycin on DPBS (PanEco).

Techniques: